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MARBIONC ELISA Kits are now available through SeaTox Research and can be purchased following the link here or reaching out to UNCW for a direct referral.
Brevetoxin/Ciguatoxin Test Kit
We offer a competitive receptor binding assay kit for the site 5 sodium channel ligands brevetoxin or ciguatoxin. This kit can detect and quantify either of these toxins ¹,². The kit can be used as a screening or quantitative tool with analyses completed in less than 2 hours after extraction. This methodology is faster and less expensive that LC/MS or N2A cell-based methods.
Each kit contains assay buffer, mixing plate, filter plate Brevetoxin standard, fluorescent ligand and receptor (synaptosomes).
Detailed instructions and a list of supplies to acquire and instrumentation settings are provided.
Each kit is capable of analyzing from 5 to 8 samples in dilution series (quantative mode) or up to 40 samples at a single dilution in duplicate (qualitative mode).
1. McCall, et al, 2012, “Development of a Competitive Fluorescence-based Synaptosome Binding assay for Brevetoxins”, Harmful Algae 19, 85-91
http://www.sciencedirect.com/science/article/pii/S1568988312001114
2. Litaker, et al, 2014, “Ciguatoxin concentrations in invasive lionfish estimated using a fluorescent receptor binding assay”, 16th ICHA Proceedings, 184-189. https://www.researchgate.net/publication/282766541_Ciguatoxin_concentrations_in_invasive_lionfish_estimated_using_a_fluorescent_receptor_binding_assay
3 - Hardison, et al, 2016, “Fluorescent receptor binding assay for detecting ciguatoxins in fish”, PLoS ONE 11(4): e0153348. doi:10.1371/journal.pone.0153348 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0153348
* A product of SeaTox Research, Inc.
Each kit contains assay buffer, mixing plate, filter plate Brevetoxin standard, fluorescent ligand and receptor (synaptosomes).
Detailed instructions and a list of supplies to acquire and instrumentation settings are provided.
Each kit is capable of analyzing from 5 to 8 samples in dilution series (quantative mode) or up to 40 samples at a single dilution in duplicate (qualitative mode).
1. McCall, et al, 2012, “Development of a Competitive Fluorescence-based Synaptosome Binding assay for Brevetoxins”, Harmful Algae 19, 85-91
http://www.sciencedirect.com/science/article/pii/S1568988312001114
2. Litaker, et al, 2014, “Ciguatoxin concentrations in invasive lionfish estimated using a fluorescent receptor binding assay”, 16th ICHA Proceedings, 184-189. https://www.researchgate.net/publication/282766541_Ciguatoxin_concentrations_in_invasive_lionfish_estimated_using_a_fluorescent_receptor_binding_assay
3 - Hardison, et al, 2016, “Fluorescent receptor binding assay for detecting ciguatoxins in fish”, PLoS ONE 11(4): e0153348. doi:10.1371/journal.pone.0153348 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0153348
* A product of SeaTox Research, Inc.